Lsof for windows : Process Explorer

Start process Explorer ( V16.02 ) 
C:\tools> procexp
Use process explorer and find 
Find Handle or DLL supstring : grac41.vdi
VirtualBox.exe    VirtualBox.exe        136.112 K    100.040 K    1972    Oracle VM VirtualBox Manager    Oracle Corporation
  VirtualBox.exe    5068    File    E:\VM_HOME\GRAC11204\grac41.vdi
    File    E:\VM_HOME\GRAC11204\grac41.vdi

Note with process explorer you close this file 
-->In the file Handle window press right mouse button and select : CLOSE HANDLE

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